Identifying the mechanism of influence of factors of different origin on the morphogenesis of the heart in the process of postnatal ontogenesis and the spectrum of pathological conditions is multistage, so determining the general patterns of cardiovascular disorders in the experiment is one of the main tasks of modern biology and medicine.
According to the literature, the effect of some chemicals and electromagnetic radiation, in general, has been experimentally determined, but the effects of certain frequencies of electromagnetic radiation on cardiac morphogenesis, especially in hypothyroidism after thyroidectomy and the relationship of this condition to electromagnetic radiation, remain unexplored. This justifies the relevance of this study and the feasibility of its conduct.
Our research aimed to study the morphological patterns of morphogenesis of the heart and structural components of the myocardium of experimental animals under the influence of electromagnetic radiation in normal and after thyroidectomy.
The study included 126 hearts of laboratory adult rats, which were divided into 6 groups; control group; 2nd group after exposure to ultra-high frequencies of electromagnetic radiation with an exposure of 45 minutes; 3rd group after exposure to ultra-high frequencies of electromagnetic radiation with an exposure of 120 minutes 21 samples; 4th group after thyroidectomy; Group 5 after thyroidectomy and exposure to ultra-high frequencies of electromagnetic radiation with exposure for 45 minutes; Group 6 after thyroidectomy and exposure to ultra-high frequencies of electromagnetic radiation with an exposure of 120 minutes.
For the first time a complex morphological, general histological, immunohistochemical study of the fine organization of structural components of the myocardium of rats after exposure to ultrahigh frequencies of electromagnetic radiation with different exposure times, both independently and in experimental hypothyroidism. Under the conditions of modeling hypothyroidism after thyroidectomy in the myocardium destructive changes of muscle fibers are observed, but after exposure to ultrahigh frequencies with exposure of 45 minutes comes regenerative compensatory rearrangement of structural components of the myocardium of the heart wall, after thyroidectomy, and exposure to microwave radiation. destructive processes in the myocardium.
For the first time, a comparative ultramicroscopic study using electron microscopy was performed, which showed that after electromagnetic radiation with an exposure of 45 minutes, the overall structure of the mitochondrial apparatus does not change. Radiation exposure for 120 minutes destroys subsarcolemmal and para nuclear organelles, edema, and degradation of intermyofibrillar mitochondria. After exposure to electromagnetic radiation in the exposure for 45 minutes under conditions of hypothyroidism, there is a compensatory adjustment of the energy apparatus of the contractile cardiomyocytes of the left ventricle due to the formation of mitochondria and increased contact interaction. After the action of irradiation for 120 minutes, the development of destructive degenerative processes in the mitochondrial apparatus of left ventricular cardiomyocytes, deepening of damage to intramitochondrial contacts is observed.
After immunohistochemical study using monoclonal antibodies to CD-34. for the first time there were changes in the expression of CD-34 in the endothelium of the vessels of the endocardium after exposure to electromagnetic radiation with an exposure period of 120 minutes compared with 45 minutes; decrease in CD-34 expression in the nuclei of myocardial cells after exposure to electromagnetic radiation with an exposure period of 120 minutes under conditions of thyroidectomy and increase after exposure to electromagnetic radiation with an exposure period of 45 minutes under conditions of thyroidectomy. For the first time, the importance of the distribution of this marker after exposure to electromagnetic radiation, both independently and in conditions of hypothyroidism after thyroidectomy, and not only its expression, was stated.
The results of the study make it possible to predict the effects of the impact on the structural components of the myocardium during thyroidectomy and the correction of this condition after exposure to electromagnetic radiation with a certain period of exposure. The obtained results on the morphogenesis of the heart after exposure to electromagnetic radiation with different exposure times, both independently and in hypothyroidism after thyroidectomy of experimental animals allow us to get an idea of morphogenetic changes in the myocardium and blood circulation, which we can predict and correct. especially taking into account the exposure period - 45 and 120 minutes.
