The dissertation is devoted to the study of toxicity parameters, pharmacological and antimicrobial properties of the newly created drug «Vitosept» and its effectiveness in pre-incubation treatment of chicken eggs and treatment of infected wounds in animals. Vitosept is based on a solution of high-purity sodium hypochlorite, obtained in a specially designed diaphragm-free flow cell, by a direct electrochemical reaction, bypassing the formation of molecular chlorine. An isotonic solution of sodium chloride (0.9% NaCl) prepared on water purified by a special technology was used as the starting electrolyte. The solutions do not contain impurities of organic substances and ions of transition metals.
Vitosept has been found to be a low-toxic drug. Intragastric administration of the drug to laboratory rats and intraperitoneal administration of the drug to white mice at a concentration of 1000 mg/l did not cause death. No violations of physiological processes in their body were observed.. According to Ukrainian Organization Standard (UOS) 85.2-37-736: 2011 the investigated means belongs to the 4th class of toxicity (low-toxic substances). The studied hematological parameters were within physiological values.
The drug «Vitosept» in the studied doses had a stimulating effect on protein synthesis.
Examining the functional state of individual organs and systems, it was found that the antitoxic function of the liver in experimental animals of the third and fourth groups, who were given a hypochlorite-containing drug at a concentration of 100 and 500 mg / l, was 1.6 and 2 times lower compared to control. Similarly, the behavioral responses of these animals according to the "open field" test and the level of their physical capabilities were dependent on the concentration of Vitosept. With increasing dose of the drug and the duration of its receipt, there were also some characteristic functional abnormalities in the body of experimental animals. Thus, against the background of growth on the 20th day of the experiment in the serum of animals of the fourth group of aspartate aminotransferase activity, the De Ritis coefficient tended to decrease. The suppression of the functional state of the liver and kidneys in animals of this experimental group also indicated a decrease in their blood concentration of bilirubin direct by 46.2%, urea - by 5.1% and creatinine - by 8.9%, in accordance. In addition, it was found that rats treated with Vitosept at a concentration of 100 and 500 mg / l decreased protein synthesis.
As a result of the conducted researches it was established that the use of different concentrations of the drug «Vitosept» in rats during 30 days before and during pregnancy does not have embryotoxic and teratogenic effects. Evaluating the antimicrobial action of hypochlorite-containing biocidal product, it was confirmed that Vitosept has pronounced bactericidal, sporocidal and fungicidal effects. It was found that the disinfectant effect is provided by pre-incubation treatment of chicken eggs with the drug «Vitosept» at a concentration of 500 mg / l. Under these conditions, the hatchability of chickens, compared with a batch of eggs treated with formaldehyde vapor, increases to 97.5%, and safety - up to 95.7%.
The antiseptic activity and efficacy of the study agent were determined in the treatment of experimental wounds in rats and infected wounds in dogs. In the conditions of simulated stencil wounds it was found that the speed of healing and reduction of the area of experimental wounds in rats of experimental groups, the drug «Vitosept» was not inferior to the effectiveness of the drug of choice «Dioxyzol-Darnitsa». In clinical trials of Vitosept in dogs, it was found that it provides effective antimicrobial and wound-cleansing action in the treatment of infected wounds, promotes the development of granulation tissue in the wound and its epithelialization.
The scientific novelty is confirmed by the declaratory patent for the utility model №144831 «Method of pre-incubation treatment of eggs».
Based on the research results, the Technical Specification of Ukraine 21.2-02070758-001: 2020 for the newly created drug «Vitosept» was obtained.
Key words: high-purity sodium hypochlorite, Vitosept drug, toxicological parameters, pre-incubation treatment of eggs, laboratory animals, dogs, infected wounds.
