Khropot O. The use of plants of the family Ranunculaceae to obtain biologically active substances in the in vitro system

The dissertation is devoted to obtaining the callus biomass of Anemone nemorosa L., Delphinium elatum L., Pulsatilla alba introduced into a culture in vitro, their complex phytochemical study, and determination of their pharmacological activity. According to the analysis of scientific publications, plants of the Ranunculaceae family, in particular, Anemone nemorosa L., Delphinium elatum L., Pulsatilla alba Reichenb. (Pulsatilla scherfelii) contain a significant amount of biologically active substances and characterized by numerous pharmacological activities. These plants have long been used in folk medicine and are promising raw materials for the production of phytopreparations. However, these medicinal plants are rare species, stocks of their raw materials are limited, and medicinal preparations based on A. nemorosa L., D. elatum L., and P. alba Reichenb. are absent in the Ukrainian market of drugs. An alternative source of biomass and BAS produced by these plants is in vitro cultivation. The use of the cell culture method makes it possible to regulate the callus biomass accumulation, optimize growing conditions to increase the amount of the target product, as well as to standardize the composition of both medicinal raw materials and finished phytopreparations. For the first time for a plant of the family Ranunculaceae: Anemone nemorosa L., Delphinium elatum L., and Pulsatilla alba Reichenb., biotechnological approaches to the production of BAS in vitro have been developed and scientifically substantiated.