Hrytsyk R. The investigation of pharmacological properties of Artemisia L. extracts

The thesis presents the results of pharmacological studies of Artemisia absinthium L. and Artemisia vulgaris L. extracts. The effectiveness of using of Artemisia absinthium L. dry extract (extractant – 70% ethanol) as a choleretic, hepatoprotective and anti-inflammatory agent and Artemisia vulgaris L. dry extract (extractant – 70% ethanol) as a gastroprotective and sedative agent was experimentally substantiated. The non-toxicity of extracts was proved on the basis of toxicological researches. It indicates the absence of toxic effects of extracts in this dose and characterizes them as approximately non-toxic (V toxicity class, LD50> 5000 mg / kg) according to the classification of substances by toxicity. The chronic toxicity of Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts was experimentally studied. It was found that the using of the studied extracts during 6 months did not cause the death of animals. While long-term using of Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts, no changes in vital systems were observed, that indicates the absence of toxic effects on homeostasis. It was found that Artemisia vulgaris L. herb extract at a dose of 50 mg / kg showed better antiulcer activity (67.45%) in comparison with Artemisia absinthium L. herb extract (42.35%), but it was slightly lower in comparison with a drug "Altan" (75.29%). The study of hepatoprotective activity was performed using a model of acute carbon tetrachloride hepatitis. The results showed that Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts in acute toxic liver disease have a pronounced hepatoprotective activity. We conducted the comparative study of the anti-inflammatory activity of Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts using three models of inflammation caused by different alternative factors (carrageenan, formalin and zymosan edema). To study the dose-dependence of the choleretic effect, the extracts of Artemisia absinthium L. herb and Artemisia vulgaris L. herb were used in doses: 25 mg / 100 g; 50 mg / 100 g; 100 mg / 100 g. The strongest choleretic effect was observed with the introduction of Artemisia absinthium L. herb extract at a dose of 50 mg / kg of animal weight; bile secretion increased by 64.6%, which is 1.64 times higher than in the control group of animals and exceeded the comparison drug "Flamin" by 19.2%. Studies of antianxiety and antidepressant effects of Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts were carried out. The extract of Artemisia vulgaris L. herb revealed the most pronounced orienting-research activity at a dose of 25 mg/kg. While the study of antimicrobial activity, it was found that Artemisia L. herb extracts including Artemisia absinthium L. herb and Artemisia vulgaris L. herb extracts show antimicrobial effect against rod-shaped, coccal microflora and yeast-like fungi. 70% and 90% ethanol extracts of Artemisia L. herb inhibited the growth of both gram-positive (staphylococci, streptococci, Enterococcus faecalis, Propionibacterium acnes) and gram-negative bacteria (Esherichia coli, Klebsiella ozaenae, Citrobacter freundii, Pseudomonas aeruginosa)