At first time on the regional level it was detected and statistically confirmed that the main cause of community-acquired pneumonia in children older than 5 years of age was Streptococcus pneumoniaе (that was isolated from the pharynx – 41.9 %, p=0.004; sputum – 49.9 %, p=0.005), in children less than 5 years of age – β - haemolytic streptococci group А were the main group of microorganisms (pharynx –52.9%, p=0.002). In children with acute bronchitis more than 5 years of age prominent pathogens were β - haemolytic streptococci group А (pharynx – 42.9 %, p=0.015) and Staphylococcus aureus (sputum – 45.5 %, p=0.03), children with acute bronchitis less than 5 years of age were most commonly found to have Staphylococcus aureus (pharynx – 40.0 %, p=0.014).
Scientific information about adhesive properties of microorganisms, causing respiratory infections in children was expanded. It was proved that Index of adhesion of Gram-negative microorganisms, isolated from children with community-acquired pneumonia was higher comparing with those, isolated from children with acute bronchitis, (р<0.05).
It was statistically proved that adhesive properties of Gram-negative microorganisms were higher in comparison with Gram-positive microorganisms (р<0.05), which can be explained by the presence of bigger amount of adhesions in Gramnegative microorganisms.
Scientific information about adhesive properties of Pseudomonas aeruginosa was clarified. Index of adhesion of Pseudomonas aeruginosa strains, isolated from children with community-acquired pneumonia, was the highest in comparison to other microorganisms (H=14.35; р<0.05).
It was proved that density of primary biofilms, causative agents of respiratory infections in children, was higher in comparison with secondary biofilms (Z=8.55; p<0.05).
It was established that density of primary biofilms, formed by Gramnegative microorganisms causing community-acquired pneumonia in children, was bigger than density of biofilms, formed by Gram-negative microorganisms causing acute bronchitis in children (p<0,05).
For the first time it was detected that primary and secondary biofilms, formed by Gram-negative microorganisms causing community-acquired pneumonia in children, were thicker than biofilms, formed by Gram-positive microorganisms.
The study for the first time revealed that optical density of secondary biofilms formed by Gram-positive microorganisms in children with acute bronchitis was significantly higher in comparison with optical density of secondary biofilms formed by Gram-positive microorganisms isolated from children with community-acquired pneumonia (p<0.05).
The maximal and minimal optical density of planktonic cells isolated during the formation of primary biofilms by microorganisms causing community-acquired pneumonia was found for strains of Klebsiella pneumoniaе and Pseudomonas aeruginosa, respectively (H=20.39; p=0.0001). On the other hand, maximal and minimal optical density of planktonic cells isolated during formation of secondary biofilms by microorganisms causing acute bronchitis were shown to be among representatives of Gram-positive microorganisms, namely Staphylococcus aureus and Streptococcus pneumoniae, respectively (H=17,44; p=0,0002); that could help in the formation of the next generations of biofilms.
The study revealed reverse correlations when using clarithromycin (R=-0.65, p=0.02) on the formed primary biofilms of Staphylococcus aureus, ceftriaxone (R=-0.77; p=0.003) and amykacin (R=-0.63, p=0.03) on the formed primary biofilms of Klebsiella pneumoniae;clarithromycin (R=-0.76; p=0.005) and levofloxacin (R=-0.78; p=0.003) on the secondary biofilms of Pseudomonas aeruginosa, that can be explained by the fact that therapeutic doses of antibacterial agents stimulated growth of biofilms of microorganisms.
An algorithm of predicting antibiotic resistance on the base of detection ability of microorganisms causing respiratory infections in children to form biofilms, results of sensitivity of these microorganisms in biofilms to the action of antibacterial agents and molecular-genetic method of plasmids detection was suggested.
