The dissertation is devoted to studying the state of immune and antioxidant potential of dogs under the conditions of toxocariasis and the action of corrective factors.
New complex data characterizing the state of the immune and antioxidant systems of the dog's body under the conditions of experimental toxocariasis and the action of the drugs «Fenbendazole» and «Fenbenzyl» were obtained. The dynamics of indicators of enzymatic and non – enzymatic components of the system of antioxidant defense, immune system, protein-synthesizing function, and functional state of the liver of dogs in experimental toxocariasis was studied. The functional relationship between the immune potential and the activity of the antioxidant defense system and the intensity of lipid peroxidation processes in canines under the conditions of Fenbendazole and Fenbenzyl has been proved and analyzed.
A comprehensive assessment of morphological and biochemical parameters of dogs' blood makes it possible to comprehensively assess the degree of damage to the body by toxocariasis and choose appropriate methods of correction of the protective systems of animals.
Infected animals recorded impaired protein-synthesizing function of the liver and functional liver condition.
In examining the antioxidant system, it was found that catalase and superoxide dismutase activity on the 5th day of the experiment increased slightly. Still, subsequently, the activity of these enzymes in the blood of dogs infected with toxocariasis decreased. A decrease in the glutathione link was observed, which reduces the level of reduced glutathione and enzymes: glutathione reductase and glutathione peroxidase.
The invasion of dogs with toxocariasis caused an immunosuppressive effect, as indicated by a reduction in all levels of immune defense.
A new complex drug, «Fenbenzyl» was developed, and the Technical Conditions of Ukraine were approved. It is theoretically substantiated and practically proved that the administration of the drug «Fenbenzyl» to dogs for toxocariasis promotes rapid recovery of hematopoietic, antioxidant immunobiological functions, and the functional state liver and its protein-synthesizing function.
The use of Fenbenzyl in dogs under conditions of experimental toxcoarosis had a normalizing effect on protein metabolism, including an increase in total protein, albumin fraction, and decrease in globulin, as well as the restoration of the albumin-globulin ratio. At the same time, a decrease in the activity of the studied transaminases was recorded in the blood serum of dogs against the background of experimental toxocariasis.
The use of fenbenzil in infected dogs contributed to a more likely increase in the antioxidant status of the dogs of the second experimental group, as this drug includes milk thistle, which exhibits antioxidant properties due to the presence of silymarin, which repairs damaged cells.
Feeding Fenbenzil to dogs with experimental toxocariasis caused a normalizing effect on the body's immune function.
Studies have shown that using the drug «Fenbenzyl» has a more significant effect on restoring antioxidant and immune potential in dogs with experimental toxocariasis than the administration of the drug «Fenbendazole». The drug «Fenbenzyl» components supplement the prescribed therapy and, under the conditions of concomitant use in toxocariasis in dogs, show high therapeutic efficacy.
The scientific novelty of the obtained results is that a comparative analysis was performed for the first time. The state of cellular, humoral, and nonspecific immune systems, the intensity of lipid peroxidation, the activity of antioxidant defense system, and functional state of the liver in dogs under experimental toxocariasis and actions of the studied corrective factors were studied. We have developed a new drug, «Fenbenzyl», made based on fenbendazole and milk thistle. The expediency of using the drug «Fenbenzyl» in dogs with the development of toxocariasis invasion has been scientifically substantiated and experimentally confirmed. The corrective effect of Fenbenzyl on the state of the immune and antioxidant systems, the intensity of lipid peroxidation processes, protein-synthesizing function, and the functional state of the liver of dogs under the conditions of toxocariasis have been experimentally proved.
Key words: parasitology, pharmacology, dogs, toxocariasis, immune system, antioxidant system, drug «Fenbendazole», «Fenbenzyl».
