Martynenko R.V. MORPHOLOGICAL AND FUNCTIONAL CHARACTERISTICS OF MONOCYTIC SPROUT AND CELLS OF THE RED BONE MARROW AFTER TRIPTORELIN ADMINISTRATION IN RATS.
Currently, the morphological and functional features of red bone marrow (BM) are of great interest to the world scientific community, as it acts as a central organ of hematopoiesis and immunopoiesis. It is known, that the red bone marrow begins its functioning during the prenatal period of human development, and after the birth it serves as the only source of regeneration of blood cells. In the course of studying scientific literature, our attention was drawn to the peculiarities of the course of monocytopoiesis. It is known that the maturation cycle of cells of this lineage is completed in other tissues, where monocytes turn into macrophages. The bone marrow microenvironment consists of many cell types, and the relative contribution of each of these cells to the haematopoietic clone support is still highly unclear.
The aim of the study was to investigate the morphological and functional characteristics of monocyte sprout and red bone marrow surrounding cells after administration of triptorelin and correction of this condition with quercetin in rats.
The dissertation provides a theoretical generalisation and ways to of solving the actual scientific problem, which is to study the reaction of structural components of monocyte sprout and red bone marrow environment cells in rats to the introduction of triptorelin, which leads to deprivation of hormone synthesis, in particular luteinizing hormone and follicle-stimulating hormone, with the subsequent correction of this condition by the introduction of the carbohydrate-modified drug quercetin.
For the first time, with the help of adequate morphological research methods, a comprehensive anatomical, histological and morphometric characteristic of the monocyte sprout and red bone marrow surrounding cells in the control group of rats, with the administration of triptorelin and triptorelin with quercetin were obtained.
The understanding of morphological and functional differences in the quantitative and qualitative cellular composition of the monocyte sprout and cells of the red bone marrow environment, which allows to analyse and compare the changes that occur in the red bone marrow during experimental research.
It has been proven for the first time that subcutaneous injection of triptorelin acetate causes multidirectional changes in monocyte sprout cells and cells of the red bone marrow, which is confirmed by the results of our research. It was found that the reaction of the monocyte sprout to the administration of triptorelin has a one-way direction and synchronous changes in both quantitative and qualitative indicators. The corrective effect of quercetin administration was found, which is confirmed by significant changes in the structural organisation and quantitative composition of cells.
Key words: red bone marrow, myeloid sprout, monocyte sprout, monocyte, macrophage, dendritic cell, cells environment, oxidative stress, triptorelin, quercetin, hormone, testosterone, luteinising hormone, hormonal dysregulation, Ki67, CD68, rats.
