The thesis provides a theoretical generalization and a new solution to the actual scientific problem, which is to compare the effect of acute blood loss in the amount of 1 and 2 % of the rat's weight on metabolic, functional and structural state of the kidney after 1, 3 and 7 days of posthemorrhagic period and to prove the higher efficiency of combination therapy with Ringer's lactate and 2-ethyl-6-methyl-3-hydroxypyridine succinate in the correction of acute kidney injury compared to monotherapy with a balanced crystalloid. It was shown that under the influence of acute blood loss of varying severity in the kidney, the activity of lipid peroxidation processes increases, which is accompanied by an increase in the content of primary and secondary products after 1, 3 and 7 days of the experiment compared to the control. Acute blood loss in the amount of 2 % of the rat's weight causes a greater increase in the value of the studied parameters starting from day 1 of the experiment.
It was found that modeling of acute blood loss in comparison with the control causes impairment of the enzyme and glutathione links of the antioxidant defense of the kidneys. Under conditions of acute blood loss in the amount of 2 % of the rat's weight, from 1 to 7 days of the experiment, the superoxide dismutase and catalase activities and the content of reduced glutathione gradually decrease in the kidney, glutathione peroxidase and glutathione reductase activity, whereas against the background of acute blood loss in the amount of 1 % of the rat's weight, most of the antioxidant defense indicators return to the level of the control group by day 7 of the experiment. It was found that modeling of acute blood loss compared to the control causes a significant decrease in diuresis, glomerular filtration rate, increase in serum and urine creatinine levels, and decrease in creatinine clearance. Under conditions of acute blood loss in a volume of 2 % of the rat's body weight, the violation of the studied parameters deepens by day 7 of the experiment and is statistically significantly greater than after modeling acute blood loss in a volume of 1 % of the rat's body weight. It was found that acute blood loss causes a violation of the ion-regulating function of the kidneys, which is proportional to the volume of blood loss and increases from day 1 to day 7 of the experiment. Under these conditions, compared to the control, the minimum level is reached by the content of sodium ions in the blood serum, sodium filtration, proximal and distal sodium transport, and clearance of sodium-free water, and the maximum level is reached by the content of sodium ions in the urine, The content of potassium ions in the blood serum and urine, urinary protein and their excretion increased, which at all times of the experiment were statistically significantly higher than in the control and compared to rats with acute blood loss in the volume of 1% by weight. It was proved that the use of Ringer's lactate solution in combination with 2-ethyl-6-methyl-3-hydroxypyridine succinate in rats with acute blood loss in a volume of 2 % by weight, compared to rats without correction, after 3-7 days of experiment, helps to reduce the activity of lipid peroxidation processes, enhance the enzyme and glutathione links of antioxidant defense, and improve the functional state of the kidneys. It was shown that the effectiveness of the combined use is significantly higher than after monotherapy with Ringer's lactate solution.
