In the course of the research, the method of isolation of probiotic microorganisms was worked out and improved. During 2021-2023 honey collection season (from May to September), 120 samples of different botanical composition and territorial placement of apiaries were selected from the honey received for research. Samples of different storage periods (the first, second, third and fourth day after pumping) were prepared from the experimental samples, from which bacteria of the species Lactobacillus plantarum, Bifidobacterium bifidum and Enterococcus faecium, characteristic of the gut microbiome of bees, were isolated by means of microbiological studies. It was established that for the isolation of the specified probiotic microorganisms it is advisable to use freshly pumped honey delivered to the laboratory no later than 3 days after pumping. As a result of the study of cultural, morphological and biochemical properties, it was established that the isolated microorganisms belong to three species, namely Lactobacillus plantarum, Bifidobacterium bifidum and Enterococcus faecium. The result of the conducted experiments is the establishment of the fact of the absence of pathogenic microorganisms of the Salmonella species in the test samples. The number of molds and yeasts met the norms of the current legislation for food products, including honey. Therefore, the selected honey samples can be used for further research. The lack of growth of Streptococcus pluton in the presence of bacteria of the genera Lactobacillus, Bifidobacterium and Enterococcus has been experimentally proven. Along with this, signs of growth retardation of Bacillus subtilis and Escherichia coli were found under the condition of cultivation in the presence of bacteria of the species Lactobacillus plantarum, Bifidobacterium bifidum and Enterococcus faecium. The results of the research prove the antagonistic properties of the selected microorganisms in relation to representatives of the conditionally pathogenic microflora of the gut of bees, as well as the causative agent of european rot. The specific probiotic for bees "Apinormin" registered in Ukraine was chosen as a model sample for further research. The use of the probiotic "Apinormin" for the treatment of bees bodies in selected dosages (0.4 g/family; 0.8 g/family, 4 g/family and 8 g/family) was not toxic to bees. The highest survival rate in the experiment was demonstrated by a group of insects treated with the probiotic "Apinormin" at a dose of 4 g/family, an average of 4 insects. Using the probiotic "Apinormin" as a supplement slowed down the dynamics of their natural death. The largest number of insects came out of the experiment in the group receiving "Apinormin" at a dose of 8 g/family, namely, an average of 9 insects. The fewest bees survived in the control group, namely 2 insects. The survival rate of insects in experimental groups treated with "Apinormin" at a dosage of 0.4 g/family averaged 3 insects, 0.8 g/family - 3 insects, 4 g/family - 6 insects. Based on the analysis of the obtained results, the optimal dose and method of application of the probiotic "Apinormin" for bees were chosen for further research, namely 4 g/family as supplement. The use of the drug " Anolite " as a supplement for bees caused an acceleration of the dynamics of the natural death of insects by an average of 5%, compared to a similar indicator in the control group. At the same time, the dynamics of natural death of bees in experimental group 3, which received a combination of the probiotic "Apinormin" and the drug " Anolite " slowed down on average by 27% compared to the control and by 11% compared to group 1, which received the probiotic "Apinormin" in a dose of 4 g/family. The obtained results indicate the expediency of using the drug " Anolite " to activate the probiotic "Apinormin" as a supplement for bees. In order to experimentally substantiate the expediency of using a specific probiotic for beneficial insects, a study was conducted to determine the effect of probiotics of various purposes on the dynamics of the natural death of bees. For this purpose, a number of probiotic preparations were selected, in particular the probiotic for bees "Apinormin", the probiotic preparation "Bazhana", the probiotic "Purina ProPlan FortiFlora" and the enzyme-probiotic preparation "Immunobacterin D". Indicators of the dynamics of the natural death of bees when using the listed probiotic preparations as a supplement at a dose of 4 g/family showed an acceleration of the death of insects in groups where the drug "Purina ProPlan FortiFlora" was used by 2%. In the group where "Immunobacterin D" was used for feeding at various stages of the study, the dynamics of the death of bees accelerated and slowed down by an average of 3%, that is, the effectiveness and expediency of the use of the drug cannot be traced.
