Lugovoy S. Сryopreservation effect on thyroid gland cell culture from newborn piglets

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0404U000342

Thesis Registration Form

0404U000342.pdf

Applicant for

Specialization

  • 14.01.35 - Кріомедицина

23-12-2003

Specialized Academic Board

Д 64.242.01

Institute of Cryobiology and Cryomedicine, National Academy of Sciences of Ukraine

Essay

In work the comparative analysis native and cryopreserved of organ cultures of thyroid gland from newborn piglets is carried out depending upon the conditions of introduction and DMSO concentration, as well as freezing rate. We concluded the expediency of the use of 7% DMSO concentration while added under 4 C. The maximum integrity of endocrine material is provided by two-step freezing rate with 10 C/min up to -80 C with the subsequent immersion into liquid nitrogen. It was determined that under the conditions of reculturing the cryopreserved organ culture of thyroid gland from newborn piglets kept the ability to basal and stimulated secretion of thyroid hormones. Storage in low-temperature bank upto two years does not cause the authentic changes of functional characteristics of thyroid gland organ culture from newborn piglets. Transplantation of cryopreserved organ cultures of thyroid gland from newborn piglets to the animals with experimental hypothyroidism resulted in normalizing effectof the hormonal status of those.

Read more

Files

all_dissertation_pictures.doc

all_dissertation_pictures.doc

aref.doc

ВВЕДЕНИЕ.doc

ЗАКЛЮЧЕНИЕ.doc

РАЗДЕЛ 1.doc

РАЗДЕЛ 2.doc

РАЗДЕЛ 3.1.doc

Раздел 3.2.doc

Раздел 3.3.doc

Раздел 3.4.doc

Раздел 3.5.doc

Раздел 3.6.doc

СПИСОК ЛИТ.doc

Similar theses

0421U101272

Vlasov Oleksandr Oleksiiovych

Effect of extract of cryopreserved piglet`s skin fragments and fullerene C60 on alterative and regenerative processes in skin after cryodestruction (experimental study)

0421U101128

Vlasov Oleksandr

Effect of extract of cryopreserved piglet`s skin fragments and fullerene C60 on alterative and regenerative processes in skin after cryodestruction (experimental study)

0420U101007

Likhitskyi Oleksii Oleksiyovych

The use of cryopreserved placental tissue for correction of the reparative osteogenesis processes of the lower jaw during an angular fracture with osteoporosis (experimental study)

0420U100468

Aidarova Victoriia Serhiivna

Effect of rhythmic hypothermia and cryopreserved cord blood on structural-functional state of central nervous system of rats with dyscirculatory encephalopathy (experimental study).

0419U003851

Chub Oleg V.

Effect of cryopreserved placental explants on course of postmenopausal dyscirculatory encephalopathy (experimental study)