Lepeeva E. The state of adrenoreceptor complex and tumor necrosis factor-a at hypertension

Object of the study: hypertensive patients with left ventricular remodelling Purpose of the study: to determine role of left ventricular remodelling factors - catecholamines, adrenoreceptor functional activity, secondary messengers and tumor necrosis factor-a - in pathogenesis of hypertension and development of myocardial structural-functional changes for improvement of hypertensive heart diagnostics. Methods of the investigation: Content of epinephrine and norepinephrine was estimated by spectrofluorimetric method. Functional state of adrenoreceptor complex was learned by EHF-dielectrometry method using specific receptor agonists and antagonists. cAMP and cGMP levels and TNF-a concentration were measured by immunoenzyme method. Structural-functional parameters of heart were diagnosed by ultrasound method. Theoretical and practical results: The following studies substantiated expediency to determine content of epinephrine and norepinephrine, adrenoreceptor functional activity of platelets, cAMP and cGMP concentration and TNF-a level as the factors which expose risk of development heart changes in hypertension and prevent complications. The measurement of these indices may be served as a diagnostic criterion of disease weight and raises quality of diagnostic to prescribe adequate treatment with due regard individual feature of each patients. Novelty: Disbalance and functional disorders of neurogumoral system, cellular and intercellular interaction are detected in hypertensive patients. The relationship between changes of functional state of adrenergic system, TNF-a and parameters of hypertensive heart is fixed. The feature of molecular platelet mechanism reactivity in hypertensive heart remodeling is evaluated for the first time. Degree of introduction: the results of the research were introduced into the work of therapy departments of hospital No. 11 (Kharkiv), therapy and cardiology departments of hospital No. 17 (Kharkiv), consultative polyclinic department of company "Medicine" (Kharkiv), Lipeskayaterritorial polyclinic (Kharkiv region).Methodical recommendations are published ("Modern aspects of diagnostics, prognosis and treatment of hypertensive heart" and "Appraisal of platelet adrenoreceptor functional state for diagnostics of hypertension complications"). Sphere of application: medicine, therapy and cardiology departments.