Naumenko O. Development of bacterial preparations technology using Lactobacillus casei ssp. casei for the production of functional milk drinks

The dissertation work is devoted to a scientific substantiation and to the development of bacterial preparations with biologically active lactic acid bacteria cultures biotechnology for functional milk drinks. The sorting through lactobacteria strains (by criteria of functional activity and technological effectiveness) from natural sources was conducted. The technique of protoplasts getting and regeneration for the selection of lactobacillus cultures with reduced acidity energy was used for the first time. New for Ukraine bacterial compositions using biologically active culture L. casei ssp. casei were created. Procedures and parametres of technological process of bacterial preparations LTS and LTS-N production for functional lactic acid drink Millenium were created. It was reached an extension of Millenium product shelf-life to 14 days by using selected acidophillus bacillus culture with reduced acidity energy. In laboratory and clinical studies it was proved functional activity of the developed bacterial preparations and products fermented by them. Industrial approbation of bacterial preparations LTS and LTS-N approved their suitability for use as preparations of direct insertion, for products high quality maintenance and their compliance with normative documentation requirements.