Dovgal A. The particularity of the development of epizootic process and control of cattle leucosis with the use of immunoferment method of diagnosis.

The object of the reseach is leukosis of large horned cattle. The goal of the reseach consists in development and introduction of the optimum scheme of investigation and improvement of large horned cattle with the use RID and ELISA tests in farms unsuccessful conserning leukosis. The goal also aims at definition the optimum terms of calves, examination by the ELISA method. The reseach methods are epizootologic, clinical, immunologic, hematologic, serologic and statistical. The scientific innovation is that for the first time using immunofermental method of diagnosis the peculiarities of calves' postcolostral immunity which were born from the leucosis cows have been investigated. Animals infected intrauterinly by leukosis virus before drinking foremilk were revealed by ELISA test-12, 5%, according to RID test -6, 2% The calves examination by the method of ELISA gives us an opportunity to remove the infected ones inneruterinly and prevent spreading of infection. Immunofermental method of diagnosis was theoretically and experimentally developed and introduced into the system of improving antileukosis measures. This method was included into draft of instruction leucosis on prevention and combating of large horned cattle what gave considerably to lift efficiency of measures for elimination of illness. We established that using ELISA in farms with the significant spreading of infection (more than 30% in our case) changes the development of epizootologic process and confirms that significant part of sick animals has the latent character of illness. The latency period varies greatly. It was marked that by using the immunodiffusion test for the diagnosis of leukosis infection the epizootical process was characterized by variability and considerable fluctuation of infecting level. The stage of fading remains practically uncompleted. Introduced: the results of research were used -while drawing and developing plans of basic measures for improvement of large horned cattle suffered from leukosis in farms in Ukraine for 1996-2000 years and 2001-2005 years; - for draft instruction on preventive measures and improvement of large horned cattle suffered from leukosis (January,2004); - while writing the appropriate sections of scientific and educational literature ; - for lectures on epizootology in profile educational institutions and refresher courses for managers and experts of veterinary medicine. Using arrear: Veterinary Medicine.