Sytnic E. Pathogenezis, diagnostics and prophylaxis of animal poisoning with Cynoglossum officinale

Object of research: The aim of the thesis was to study pathogenesis, to generalize diagnostic methods and to systematize prophylactic means of cynoglossotoxicosis in animals. Methods of research: clinical, hematological, biochemical, chemical-toxicological, patho-anatomical, statistical. Theoretical results: For the first time in Ukraine bulls acute poisoning and acute and chronic mice and rats poisoning with Cynoglossum officinale was done experimentally. The scheme of pathogenesis cynoglossotoxicosis in animals was substantiated on the basis of the received results. The disturbance of carbohydrate metabolism in horses and young of livestock which results in hyperglycemia and pyruvic acid content increase in blood and kidneys function that add to the existed idea of poisoning pathogenesis was established. It's established that an absolutely mortal dose (LD100) of a spirit infusion (1:10) of the second year vegetation Cynoglossum officinale for white mice under (the) peroral injection is 0,5 ml, the medium mortal dose (LD50) is 0,41 ml per/a head, that corresponds to the amount of dry grass 50 and 41 gr per 1 kg of body's weight. Practical results: The scheme of the diagnostics of animal poisoning with Cynoglossum officinale was offered, which includes the anamnesis data, the ration analysis, its botanic content studying, the qualitative determination of the Cynoglossum officinale alkaloids in forage, taking into account the results of the biochemical blood research for the glucose and pyruvic acid content, pathological, anatomic and histological changes of the internal organs (the brain, the heart, the kidneys, the liver, the spleen). The animal cynoglossotoxicosis prophylaxis is systematized. The results of the research are generalized in the "Methodologic recommendations in diagnostics and prophylactical measures of the animal poisoning with Cynoglossum officinale", confirmed by the veterinary medicine state department of the agrarian policy Ministry of Ukraine.