Sergutina S. Inactivation of simplex and complex model viruses in donor blood plasma (experimental investigations).

The dissertation is devoted to the study of the topical transfusiological problem, i.e. to the virus inactivation of the donor blood plasma. Оn the basis of the data got after the experimental studies on the contaminated donor blood plasma with model simple (poliomyelitis virus) and complex (vesicular stomatitis virus) viruses are worked out optimum modes of photosensitizer methylene blue (MB) and UV-irradiations combined applications for the purposes of its photodynamic virus inactivation. It is ascertained that for the most efficient blood plasma virus inactivation the MB concentration must be 10 mkmol/L within the scope of an hour of preliminary incubation with the contaminated donor blood plasma. The dose of the UV-irradiations for inactivation of the complex model viruses with the initial infectious activity of 5,33 lg TCІD50/mL, must be not less than 60 J; for inactivation simple virus with initial infectious activity of 6,83 lg TCІD50/mL it must be not less than 75 J. It is determined that elaborated modes of combined application of MB and doses UV-irradiations do not influence fractional composition of blood plasma proteins. Besides, it is established that MB concentration used for preliminary photosensitization (10 mkmol/L corresponds to 3,7 mg substance in a litre of plasma) does not exceed the dose accepted for the human (1-2 mg per 1 kg of the mass) and therefore it isn't dangerous. At the same time it is proved that the method of column chromatography with the application of TSK-gel "Toyopearl" ("Akasaka", Japan) can be effectively used for its removal from the photomodificated virus inactivated donor blood plasma. On the basis of the data obtained during the research work an experimental model of a device for the UV-irradiations of donor plasma has been designed and made ("Device for disinfection of the liquids", patent of Ukraine № 7835); the field is medicine.