Korzh A. Comparative characteristics of biochemical parameters of donor blood plasma collected through different methods

A total of 191 blood donors (97 men and 94 women) were examined: 156 active donors (81 men and 75 women) who donated plasma regularly, at least three times a year, and 35 first-time reserve donors (19 men and 16 women) who donated plasma for the first time. The dynamic of indicators of secondary metabolic disorders in the social group of active donors was studied and described, considering the type of plasma collection procedure and the intervals between donations based on biological characteristics (plasma donors). Among the examined active plasma donors, the greatest changes in the main biochemical parameters and indicators of secondary metabolic disorders (average-mass molecules, free fractions of histamine, serotonin and heparin) were found in donors of manual plasmapheresis. We have observed that in automatic plasmapheresis the changes in the main biochemical parameters of liver functional state and indicators of secondary metabolic disorders in plasma donors, based on the study of the content of average-mass molecules, free fractions of histamine, serotonin and heparin in the plasma were 1.5 times lower than in plasma collected by manual plasmapheresis method. The main iron metabolism indicators do not depend on the type of plasmapheresis, frequency and intervals between donations. Based on obtained data, we have established the diagnostic significance and the importance of determining the levels of average-mass molecules, free fractions of histamine, serotonin and heparin in donors’ plasma in order to identify secondary metabolic disorders in donors’ plasma, consideration of these parameters when admitting to subsequent donations, forming a health risk group in case these parameters’ thresholds are exceeded. It has been substantiated that the most optimal method of donor plasma collection is the use of automatic plasmapheresis, and that 14 days is the most optimal interval between procedures which does not lead to secondary metabolic disorders in donor plasma and, accordingly, ensures a proper quality of collected blood component and safety for the recipient, preventing the occurrence of non-immune febrile reactions.