Kalynychenko T. The optimization of the cryopreservation technology of the umbilicalcord blood cell content and the methodological principles of donor banks functioning

The research aim wasto improve the quantitative and qualitative safety of cryopreserved umbilical cord blood (UCB) cell suspensions by optimizing the technology of their preparing for long-term storage based on the prooxidant processes development data in the cryopreservation stages. To determine the methodological bases and principles of functioning the donor UCB banks for allogeneic clinical use as part of the national donor hematopoietic tissue (HT) registry. The research object was the cryopreservation process of the UCB cellular fractions (the nucleated cells and the red blood cells) and the organizational and functional bases for the allogeneic UCB banks. The concept of functioning the donor UCB banks is proposed, including the optimized technology, a quality control system and the bioethical principles.The cause-effect patterns of the development of increased individual sensitivity of the UCB HT to the cryopreservation factors depending on the initial parameters of the prooxidant status of a sample have been substantiated. The theoretical forecast model has been developed that allows the possible quality losses to be adequately predicted before the material processing and the amount of cryoprotection to be timely determined. It has been theoretically substantiated and practically confirmed that the individual features of the donor material associated with the presence or absence of certain membrane structures of the blood group antigens and the histo-blood group specificity determine the peculiarities of the natural limits of the UCB adaptive properties in counteracting the cryopreservation factors. On the basis of systematization and scientific substantiation of approaches to testing the HT of cryopreserved UCB, a methodology for controlling its quality and suitability for clinical allogeneic use has been developed.