Belykh I. The influence of ozone on biopolymers and ozone methods in a cryobiology

The dissertation is dedicated to analysis of the influence of different doses of ozone on biological objects. In the activity two aspects of an application of ozone in a cryobiology are investigated experimentally. The capacity of ozone in high doses for showing of an inactivating operation on microorganisms is an interest for the application of ozone as the sterilizing agent for cryobiological equipment, which one is high-gravity for sterilization by other ways. The other aspect of usage of ozone in a cryobiology is connected with a stimulant effect of small doses of ozone on a function of biological systems after freezing-thawing with the purpose of a reparation of not lethal damages. The cultivation of bacterias Escherichia coli in ozonized medium М9 at a dose of ozone of 0,12 - 0,35 mg/l results in growth of a velocity of division of bacterias in the second half of logarithmic phase and entrance in to a fixed phase at higher concentration of bacterias in comparison with control. The doses of ozoneof 0,16 - 0,64 mg/l promote a reparation of proliferative processes of yeast-like funguses Candida albicans after freezing -thawing. It is revealed, that at the presence of ozone in doses of 0,16 - 0,48 mg/l is improved stability of erythrocytes to a haemolysis in hypertonic conditions, and also in real conditions of a cryopreservation of erythrocytes. The ozone in high doses (ore than 0,3 g on 1 g of a biopolymer) provokes a destruction of a biopolymer, that can serve explanation one of possible gears of a decontamination of microorganisms by ozone. We can see that on an example of biopolymers of the ferment and not ferment nature (cholinesterase and bovine serum albumin). The whole complex of the obtained outcomes can be utilized in a cryobiology at a composition of the new modes of the cryopreservation biological objects.