Mohammed М. Influence of oxygenation of blood-substituting solution on oxidant homeostasis and state of rats' heart

Object: oxidant homeostasis in the heart tissues of rats and the functional state of the heart in substitution of profuse bleeding by oxygenated solution. Purpose: to give a scientific ground while studying the physiological mechanisms regulating the state of the heart homeostasis, to the use of oxygenated solutions with antioxidants as solutions supporting oxidant homeostasis of the heart tissues and its functional state, with the purpose of approaching these parameters to physiological in a sharp experiment. Methods: biochemical, functional, experimental. Results: it is established that in hypoxy (anoxy) the state of the heart is mainly depends not on the products of peroxidation, but on the lack of oxygen, although the full ignoring of the influence of peroxidation products is impossible, as the use of oxygenated solution with antioxidants preserved the state of the heart some better, than oxygenated solution only. Novelty: the complex study of kinetics of oxidant homeostasis in the heart tissues, its functional parameters in the first minutes after profuse bleeding and after substitution of blood by an oxygenated Ringer-Lokk solution with antioxidants was conducted for the first time; the information was obtained that under the conditions of hypoxy which accompanied a substitution of sharp bleeding by an ordinary Ringer-Lokk solution, an oxidant homeostasis is sharply broken in the first minutes; it is shown that the used antioxidants (thiotriazolyn and rumosol) when added to oxygenated solution provide a support of the functional state of the heart during six minutes of infusion, revealing the direct effect as blockers of peroxydation products; it is shown that in the first minutes of myocardial anoxy the peroxydation products are not the leading factor of violation of the functional state of the heart, but it is the deficiency of О2. It is introduced in the curriculum at the Departments of Normal Physiology of Lvov National Medical University, Ukrainian Medical Stomatological Academy, Kharkov State Medical University, Dnepropetrovsk State Medical Academy. Field: medicine.