Lam M. Pathogenic ground of arachidonic acid metabolism disorders correction at renal dysfunction after skin burn in rats with different individual reactivity

Object of the investigation - arachidonic acid metabolism after skin burn modeling, renal dysfunction and postburn nephropathy; aim - pathogenic ground of the altered arachidonic acid metabolism correction at renal dysfunction after cutaneous thermal trauma at different individual reactivity of organism; methods - physiological, morphological, biochemical, immunoenzymological and statistical methods; novelty - it was firstly established that intact rats with different adrenoreactivity have the same sensitivity of platelets PAF-receptors; the modeling skin thermal trauma didn't change sensitivity of b2-adrenoreceptors, but led to altering in PAF-receptors sensitivity and reserve power of arachidonic acid metabolism enzymes - cyclo- and lipoxygenase (COX and LOX) of monocytes. Firstly the correlation between renal function decline and PAF receptors sensitivity, COX and LOX activity was established in rats after thermal skin trauma. It base the pathogenic ground of COX and LOX inhibitors and prostaglandin Е2 administration to predict renal dysfunction after burn; results - normoreactive rats have the same reserve power of monocytes COX and LOX and have not changes in PAF-receptors sensitivity of platelets; effective restoration of renal osmoregulatory function after 1 month skin burn modeling. In hypoadrenoreactive rats renal dysfunctions were eliminated during 3 months after skin burn in conditions when elevation of PAF-receptors sensitivity and saving of the same COX and LOX reserve power of monocytes. If sensitivity of PAF receptors decreased but reserve power of COX predominates under LOX, the alteration of osmoregulatory mechanisms was stable for 6 months because of prolongation of inflammatory-reparative process in kidney. The same pattern was shown in hyperadrenoreactive rats, which demonstrate the decrease of PAF-receptors sensitivity and predomination of LOX-way of arachidonic acid metabolism. Administration of COX inhibitor Diclophenack-Sodium to rats with high reserve power of COX, LOX inhibitor zileuton to and prostenon to rats with high reserve power of monocytes LOX and decreased COX, and zileuton to animals with high LOX potency stimulated the restoration of volumo- and osmoregulatory mechanisms efficacy, intensity of transport processes in proximal renal tubules and renal ability to maximal urine concentration; it was introduced - in studding process at departments of pathophysiology of medical universities in Charkov, Zaporozhian, Crym, Ivano-Francovsk, Lugansk, Poltava stomatological academy, in practice of Institute of urgent and restoring surgery of Ukraine AMS, SRI of Traumatology и Orthopedics in Donetsk Sate medical university of Ukraine MHS.