Yakovleva O. Low temperature effect on some biophysical and biochemical characteristics of human ovary follicular fluid

The work is describing the research of cryopreservation effect on some biochemical and biophysical characteristics of human ovary follicular fluid, pro-cured from follicles of various sizes. Basing on the obtained data the author elabo-rated a cryopreservation method, involving direct plunging of samples with fol-licular fluid into liquid nitrogen. The obtained data allow to conclude that size of follicles reliably influences the composition of follicular fluid procured. Freeze-thawing of human follicular fluid and its storage at -196°C does not reliably effect the steroid hormones content and does not change the total hormonal profile of fol-licular fluid. Pro- and antioxidant balance of human follicular fluid after rapid freezing and long-term (1 year) storage at -196°C, and following hypothermic storage of frozen-thawed samples during 12 hrs at 4°C does not significantly change. Density, pH and osmotic pressure of human follicular fluid after freeze-thawing does not statistically and significantly change, while the dynamic viscosity of frozen-thawed follicular fluid increased in about 40%. Frozen-thawed follicular fluid added in 10% concentration to medium for sperm incubation statistically and significantly improves the kinetic properties of motile fractions of human sperm at astenozoospermy.