Skolozhabskyj A. Selectively cultivated hematopoietic stem cells in correction of experimental radiation-induced immunodeficiency.

Object of the research: Possibility to use pluripotent HSC in correction of immune deficiency state caused by x-ray exposure. Purpose of the research: Experimental working out of an improved technique of selective cultivation of HSC and pathogenetic substantiation of the possibility to use them fin correction of postexposure immune deficiency state. Methods of research: Hematological (amount of leukocytes, neutrophils, lymphocytes), microscopy (optical, electron microscopy) for cytometric and cytomorphological identification of SC, immunological - to determine CD34, CD90, CD117, CD133, Sca-1 specific for SC and CD4+ and CD8+, for T-lymphocytes, physiological - to determine behavioral reactions and general state of the experimental animals, parametric and non-parametric statistics. Рractical significance of the obtained findings: An improved technique of selective cultivation of homogeneous phenotypically mutation-free pluripotent HSC including autogenic which can be reproduced under the conditions of hospitals with reduction of cost of the performed procedures which opens the perspectives of a wide use of this type of cells in the clinical practice without the problems of histocompatibility. A new approach was worked out and its efficacy in treatment of radiation and possibly other immune deficiencies was shown. Novelty: For the first time, an original technique of selective cultivation of pluripotent HSC that can be used in hospitals at autogenic transplantations was suggested. A technique of intensive growing of a homogeneous culture of pluripotent HSC that can be introduced in correction of immune deficiency states of various origin, e.g. caused by x-rays, was substantiated. Degree of introduction: The findings of the research were introduced to the research work and teaching process of departments of pathological physiology of Kharkiv State Medical University, Lugansk State Medical University, Sumy State University, Odessa State Medical University and laboratory of reproductive endocrinology of Institute of Problems of Endocrine Pathology named after V.Ya. Danilevsky (Kharkiv). Area (sphere) of application: medicine, pathological physiology.