Grygorov O. Kv channels of interneurons of culture of hippocampal cells

This work is devoted to investigation of voltage-activated potassium channels in GABAergic interneurons of hippocampal culture. Using the combination of electrophysiological (voltage or current clamp in "whole cell" configuration, pair recording from the synaptically connected neurons, axon stimulation) and non-physiological (immunocytochemestry and single cell RT-PCR) techniques together with fast local superfusion technique allowed the studying of specific subtypes of potassium channels peculiar to hippocampal interneurons, the contribution of these channels to potassium currents of interneurons and their participation in regulation of the synaptic transmission. It was shown that non-inactivating current plays a main role in the forming of the integral potassium current of the soma of inhibitory interneurons. The minor contribution of the Kv2-, Kv3- and Kv4-channels to interneurons' integral potassium current was also revealed. It was proven the non-inactivation current appearance is caused by activation of channels of Kv7-family that may consist of Kv7.2, Kv7.3 and Kv7.5 subtypes of -subunits. It was display yed the presence of Kv7-channels in the somas and axons of inhibitory interneurons and demonstrated the ability of these channels to regulate membrane potentials and cells' functioning. It was conclude, first, the Kv7 channels family plays the main role in the forming of potassium currents of soma of hippocampal inhibitory cells and, second, these channels can influence on physiological behavior of interneurons and modulate the GABAergic synaptic transmission.