Ataman J. The role of lipid peroxidation in the development of arteries and veins lesions induced by primary disturbances of energy metabolism

The investigation object: mechanisms of development of dystrophically-sclerous lesions in blood vessels. The investigation goal: determination of the role of lipid peroxidation and antioxidatic systems in pathogenesis of arteries and veins lesions induced by primary disturbances of energy metabolism. Methods of research: modelling of vascular lesions by using of inhibitor of energy metabolism – monoiodacetate, biochemical methods of determining intermediate (lipid hydroperoxides) and end products (Schiff's bases) of lipid peroxidation (LPO), biochemical investigation of the activity of antioxidatic enzymes (glutathione peroxidase, superoxide dismutase, catalase), physicochemical methods of examining of water content and the inulin space volume, spectrophotometrically determining total calcium content, statistical methods. The theoretical and practical importance of the received results. Data about appreciable activation LPO in vascular tissues in conditions of monoiodacetate intoxication dilate scientific representations about mechanisms of development of dystrophically-sclerous lesions in a vascular wall. It was shown that angioprotectors with different mechanisms of action variously affected the LPO intensity and antioxidatic activity of blood vessels, on the one hand, and early manifestations of dystrophic changes in a vascular wall, on the other hand. It should be considered at a choice effective pharmacological protection of blood vessels at each concrete kind of their lesions. The obtained data can be used by working out of new approaches and means of pharmacological correction of dystrophically-sclerous lesions of blood vessels, risings of their resistance to action of different pathogenic factors. The scientific novelty of the investigation results. For the first time dynamics of LPO signs and antioxidatic activity of arterial and venous wall under condition of monoiodacetate intoxications was examined. It was shown, that monoiodacetate caused essential increase of the LPO intensity and reductionofthe activity of antioxidatic systems both in arterial and venous wall. As the tool for analysis, pharmacological agents with different mechanisms of angioprotective actions (antioxidants, blockers of calcium channels, anticalcinogenic drugs) were used. It was shown that vitamain E which decreased the LPO intensity in blood vessels did not influence early manifestation of dystrophic changes in vascular wall (oedema, calcification). At the same time the blocker of calcium channels nifedipine and anticalcinogenic drug – EHDA which did not influence the LPO indices decreased essentially oedema of vascular wall (nifedipine) and total calcium content in arterial and venous tissue (EHDA). It was shown that the levels of the LPO activation and antioxidatic system depression in venous wall were much higher than in arteries. The degree of implementation: the results of the research are implemented in the educational process at Chairs of pathophysiology of Kharkov and Crimean Medical Universities, Ukrainian Medical Stomatological Academia (Poltava), at Chair of physiology and pathophysiology of Sumy State University, at Chair of histology, cytology and embryology of Donetsk National Medical University. Branch of usage: medicine, pathophysiology.