Trusova M. Effect of cytostatic agents on the condition of renal functional reserve (experimental study)

The investigation object: state of renal functional reserve in the conditions of introduction of cytostatic of ifosfamide. The investigation goal: elucidation of renal dysfunctions pathogenesis induced by single and prolonged dosing of iphosphamide to rats as well as experimental assessment of nephroprotective features of arginine and glutargine in rats during prolonged effect of the cytostatic. Methods of research: pathophysiology: design of renal disfunctions; biochemical: determination of albumen is in urine, concentration of kreatinine, glucose, nitrites and nitrates of plasma of blood and urine; patomorfologic: research of cuts of fabrics of buds of rats; statistical: variation and cross-correlation analysis Study of nephroprotective characteristics of arginine and glutargin in rats treated with cytostatic agent iphosphamide has been performed for the first time. Single administration of the cytostatic agent to rats has been shown to result in moderate change in renal activity of the animals, manifested through the decrease of gromerular filtration rate, moderate increase of proteinuria, appearance of glucose traces in urine, retention of liquid and osmotically active substances, and intensification of renal clearance of endogenous nitrates. Prolonged iphosphamide administration to the animals has been established to result in maintenance of lower gromerular filtration rate, registered intensification of proteinuria, glucosuria, and renal clearance of endogenous nitrates, increase of malonic dialdehyde level in blood plasma and urine, and disorders of renal concentration function. The experimental results evidence that adaptive intensification of intrarenal autoregulation systems and exhaustion of systemic arginine stock plays an important role in formation of renal response to nephrotoxic effect of the cytostatic agent in pathogenesis of iphosphamide induced renal dysfunctions. The obtained results allow us to claim that arginine possesses pronounced nephroprotective activity in the conditions of prolonged iphosphamide administration, preventing the decrease of gromerular filtration rate, attenuating glucosuria and proteinuria, decreasing renal clearance of endogenous nitrites and nitrates, normalizing malonic dialdehyde level in blood plasma but not in urine, increasing concentration capacity of kidneys, and decreasing the signs of structural damage of tubular epithelium. The degree of implementation: the results of the research are implemented in the educational process at Chairs of pathophysiology of Odessa, Kharkov, Bukovinski and Ivano-Frankovsk Medical Universities.