Kliuvadenko A. The peculiarities of clonal micropropagation, direct adaptation and processing of perspective sorts of hop (Humulus lupulus L.)

The fundamental and applied aspects of the using of modern biogeotechnologies quantitative reproduction in this dissertation work, increasing of the productivity and quality of raw of perspective varieties of hop are considered. (Humulus lupulus L.). The technique of clonal micropropagation in vitro such varieties as Gaydamackiy, Kumir and National were developed with taking into account financiall and technicall support of hop industry and further development. The monitoring of hop's infection rate was done from Zhitomir region industrial hop gardens, which which shown 11% of area. The regeneration ability of different types of meristems and application efficiency in elimination of viruses were studied. The modern variation technique of biotechnological virus free material of hop production with using thermotherapy in combination with the method of apical meristem selection was proposed. The realization processes of morphogenic potential of hop tissues and organs with help of selection and modification of nutrient medium components for the different stages of morphogenesis in vitro were investigated. The ways of callus formation induction were established to the different types of hops. Using the cytological analysis of nonmorphological callus the heterogeneous structure was confirmed and meristematic zones in morphogenetic callus were determined. The influence of different concentrations of phytohormones, mineral and organic compounds of nutrient medium on induction of roots were studied. It was firstly created the direct biotechnological adaptation in vivo such variety as National that had been cloned in vitro. As result nearly 100% adapted plants were obtained, without using of hops nursery . This technology foresees the use of adaptation phytocontainers, compost and making the direct planting on industrial hop-gardens, that allows to get industrial harvests in the next year. The granulation of hop was made on minigranulator Eco-Bio 100, that allowed to get high-quality hop granules with the minimum underload losses of energy resources and valuable hop components reductant. The received granules from National variety underwent a brewing estimation appraisal and were recommended for production of selected and blended types of premium beer in Ukraine.