Semen I. Methodologica approaches to evaluation of microorganisms at constructing at probiotics

The Object: Selection of microorganisms and determination of their biological properties. The Aim: Development of methodological approaches in relation to the system of evaluation of biological proper-ties of microorganisms and establishment of parameters, necessary for legitimate confession of status of pro-biotic stamm and their fitness for probiotics constructing. Methods: Microbiological (authentication, determination of antagonism activity, protective activity, adhesive properties, microorganisms antibiotic sensitiveness), immunological (determination of bactericidal activity of blood serum, phagocyte activity of neutrophils, phagocyte number and index), mathematic-statistical analysis. Novelty: In dissertation methodological approaches in relation to the evaluation system of microorganisms bio-logical properties and search of microorganisms which will be used for constructing of an effective probiotics are worked out for the first time. Theoretically grounded and experimentally proven expediency of realization of pre-clinical researches of the studying biological properties of microorganisms, which will be used for creation of probiotics with concretely certain properties and possibilities of application. Methodological approaches are systematized in relation to the evaluation of fitness of microorganisms for creation of new probiotic preparations, which contain criteria and parameters of researches, necessary for a legitimate grant to the experienced stamm the probiotic status. Ability of probiotic stamms to promote antibiotic sensitiveness of pathogenic micrоflora on condition of their general cultivation which can warn appearance of firmness to the antibiotics is proven. With the aim of receipting effective probiotics with a certain application domain, it had been suggested to conduct testing of probiotic stamms on antibiotic sensitiveness, with the obligatory construction of antibiotic-gram that will enable to promote efficiency of treatment.