Pyeshkova V. Development of conductometric enzyme biosensors for determination of main nature saccharides

The object - enzymatic reactions that are the basis of functioning of conductometric biosensors for determination of saccharose, maltose, lactose and glucose. The aim of the work was development of new enzyme conductometric biosensors for main natural saccharides determination (sucrose, maltose, lactose and glucose) and optimization of its working characteristics for the analysis of the real samples. Methods - conductometric method (study of passing of enzymatic reactions); polarimetry; high-efficiency liquid chromatography; methods of mathematical analysis for quantitative determination of saccharides in the real samples using biosensors. Results and novelty - laboratory prototypes of conductometric biosensors for determination of sucrose, maltose, lactose and glucose have been developed as a result of research. The enzyme composition of biosensors bioselective membranes has been optimized. Analytical characteristics of biosensors have been developed and optimized. Effects of working solution parameters (pH, buffer capacity and ionic strength) and different storage conditions on functioning of biosensors have been investigated. Concentrations of sucrose and glucose have been measured with biosensors in different beverages and homogenates of sugar-beet. The results of sucrose and glucose measurement showed good correlation with traditional methods. The developed laboratory prototypes of biosensors for saccharides determination can be a prospective basis for construction and industrial production of commercial measuring devices for selective analysis of glucose, maltose, lactose and sucrose. Proposed biosensors can be applied in food, pharmaceutical industries, agriculture, enzymology and microbiology.