Khizhnya Y. The role of general and local factors in the development of calcification of the arterial wall caused by hypervitaminosis D

The investigation object - the mechanisms of calcification of blood vessels. The investigation goal - the role of common disorders of phosphorus-calcium metabolism and local changes of the vascular wall in the pathogenesis of arterial calcification, which occurs under conditions of experimental hypervitaminosis D;Methods: pathophysiologic experiment - modeling lesions of blood vessels using high doses of vitamin D.The study used a method koregiruyuschih influences (the introduction of animal EGDK for an analysis of the role of general pathophysiological disturbances phosphorecalcium obmela in the development of calcification of the arteries);biochemical methods for the determination of calcium, inorganic phosphorus, alkaline phosphatase activity in serum as indicators that characterize the general disorder of phosphorus-calcium metabolism in the body;spectrophotometric determination of content of calcium, magnesium and trace elements in bones and arteries in order to quantify disturbances of mineral metabolism in their tissues;study the biomechanical properties of bone, as well as X-ray diffraction characteristics of the samples nanodispersed bioapatita to assess the processes of resorption and demineralization of bone tissue;biochemical methods for determining the content of intermediate products of lipid peroxidation in the tissues of the aorta of experimental animals to evaluate the intensity of free radical processes as factors damaging the vascular wall;biochemical activity of antioxidant enzymes (glyutationperoksidazy, superoxide dismutase, catalase) that limit the processes of LPO in the tissues of blood vessels and prevent the development of damage to the vascular wall;biochemical methods for determining the activity of alkaline phosphatase and ecto-ATPase activity of vessels for the characterization of local pro-and antikaltsinogennyh mechanisms;physico-chemical methods of water content and volume of inulin space as indicators that characterize the early degenerative changes in the vascular wall;morphological studies of the wall of the aorta of experimental animals (hematoxylin-eosin staining, alizarin red) to identify degenerative changes and delays of calcium salts; generally accepted statistical methods for data processing. These results indicate that the changes that occur in bone, are relevant to the development of calcification of arteries and, consequently, the normalization of mineral metabolism in krovonosnyh vessels. To this end, a promising seems the use of bisphosphonates, which, on the one hand, prevent resorption and bone demineralization, and from another - reducing vascular calcification.Data obtained in this paper can be used to develop new approaches and ways of pharmacological correction of dystrophic-sclerotic lesions of blood vessels that grow under conditions of hypervitaminosis D. The results of the research outlined in the thesis, rooted in the educational process at the Department of Pathophysiology of Kharkiv, Crimea, Vinnitsa medical university, the Ukrainian Medical Dental Academy (Poltava), Department of Physiology and Pathophysiology, Sumy State University.