Abrafikova L. Effect of cryopreserved fibroblasts on reparative processes during aseptic skin inflammation in an experiment

Research objects are reparative processes during aseptic inflammation in rats after application into the zone of defect of cryopreserved and native fibroblasts. The aim of theses is comparative investigation of the effect of cryopreserved and native fibroblasts on reparative processes during aseptic inflammation of skin in rats and study of the possible deriving of fibroblasts from skin bioptates after cryopreservation and hypothermal storage. The research methods: cryobiological, morphological, biochemical, histomorphological, cell culturing in vitro, modeling of aseptic skin inflammation, methods of statistical processing of the results. It has been shown that application into the defect site of cryopreserved fibroblasts with no additional culturing provides statistically significant reduction of the terms of its healing. There is no difference in stimulating effect on reparative processes of fibroblasts prior to and after cryopreservation. There were experimentally substantiated the advantages of fibroblast immobilization in methyl cellulose gel when applying them to the skin defect. It has been revealed that dynamics of the change in inflammation acute phase indices correlates with histomorphological alterations in tissues after application to the defect of cryopreserved and native fibroblasts. The most stimulating effect of reparative processes is caused by viable cells of fibroblasts. The preparations derived from the mass of destroyed fibroblasts manifest less pronounced stimulating effect. There was demonstrated the possibility of obtaining the fibroblasts from skin bioptates after their hypothermal storage within 14 days. The composition of medium for hypothermal storage of skin bioptates with the aim of following deriving the fibroblasts was experimentally substantiated.