Golub N. Hydrolysates of the Black sea hydrobionts and their potential uses

The dissertation focuses on the methods permitting to get hydrolysates from Black sea mussels and sprat, on special features of the amino acid content, influential factors and development of the hydrolysate-based nutritional medium for culturing microorganisms. The thesis presents data having been obtained on the raw material (mollusks and fish) and the hydrolysates, and describes the effects that seasons, hydrolysis time and methods produce on the harvested raw material. Depending on harvest season amine nitrogen content of the fish and mussel hydrolysates varied from 600 to 1000 and from 105 to 270mg%, respectively. In the mussels placed in alkaline medium the hydrolysis prolonged from 3 to 24 h increases amine nitrogen content 2-3-fold compared with the control group. It was found that amino acid score of the sprat autolysate and mussel hydrolysate is amino acid-limited and that the amino acid content can be amplified to balance and approximate FAO/WHO food value standards by combining the hydrolysates. Application of intracellular cathepsins gives rise to sprat hydrolysate with high amine nitrogen content. Joined with mussel hydrolysate, it is the basal component of a growth medium which in some aspects is even better than standard media used in microbiology. Testing for cultivation optima has shown that 14 - 25% mussel hydrolysate weight injected into the sprat autolysate stimulates microbial biomass to increase 1.5-2 times compared with the biomass grown on high-quality meat media. Tests conducted on animals have proved that the mussel hydrolysate produces immunomodulatory effect, normalizes homeostasis, improves autodefense and is not toxic. The data of the investigation have underlain the documentation on the veterinary medication Migivit, the dietary additive Rapamid and the basal fraction of the nutrient medium Aquamedia.