Kryvoshapka O. The role of proinflammatory cytokines in the mechanisms of burn wound chronization

The investigation object: The object of study is thermal burns pathogenesis. The investigation goal: Toestablish the role of proinflammatory cytokines in mechanisms of burn wounds chronisationon the experimental base. Methods of research: ELISA methods of pro-inflammatory cytokines determination (IL-1 бетта, TNF- альфа, IL-8) in the peripheral blood, spectrophotometric methods of NO metabolites and SH-groups determination in the focus and peripheral blood, morphological methods, planimetric methods, morphometric methods, immunomorphological methods ofIL-1?, TNF-?, IL-8 cells-producers determining, statistical methods, parametric and nonparametric methods of the study results processing were used. Theoretical and practical value of the obtained results. Scientific novelty of the obtained results: in experimental model it was shown, that proinflammatory cytokines IL-1бетта, TNF-альфа and IL-8 influence upon the course and results of inflammatory- reparative processes of burn wound. The development of burn wound was shown to be characterized by more pronounced and longer lasting increase of marker proinflammatory cytokines IL-1бетта, TNF-альфа and IL-8 in the blood accompanied by disturbances of reparatory processes , slowing healing as compared with ordinary acute wound. For the first time performed study of cells-producents of proinflammatory cytokines IL-1бетта, TNF-альфа and IL-8 made it possible to get new data that show the significant decrease in number of the cells and their restoration in the area of burn damage and cytoprotective effect of wound healing ointments, that is proved by less decrease and faster restoration of cells producing proinflammatory cytokines in the area of damage. This investigation proved the existence of connection between proinflammatory cytokines and nitric oxide metabolites and SH-groups in peripheral blood of damaged area. The degree of implementation: The results of the thesis were embedded in the learning process of Pathological Physiology and Pharmacology Department of the National Pharmaceutical University (Kharkiv), Department of Pathophysiology of Maxim Gorky Donetsk National Medical University, Department of Physiology and Pathophysiology with a Course of Medical Biology of Sumy State University, Department of Pathophysiology of Kharkiv National Medical University.7452