Galkin O. The role of transcription factor ZXDC in molecular mechanisms regulating haemopoetic precursor differentiation

The subject of the study: transcriptional factor ZXDC as a regulator of haemopoethic precursors differentiation and gene expression. The goal: to clarify the role of ZXDC in the regulation of EGR2 gene and the other key genes involved in the differentiation of haemopoethic precursors in human promyelocyte cells with overexpression of ZXDC gene. Methods: DNA and RNA isolation, reverse transcription, PCR and quantitative PCR, cDNA and genomic sequences apmlification and cloning in plasmid vectors, luciferase reporter assay, chromatine immunoprecipitation, Western-blot analysis. Results of this study provide the information regarding the ZXDC function as a transcriptional factor in the regulation of haemopoethic precursors differentiation. In this work recombinant DNAs with cDNAs of ZXDC transcriptional factor and the other factors were created, as well as genetically modified HEK293 and HL60 cells. Obtained results regarding the gene expression regulation under the influence of ZXDC suggests the possibility of it's usage for the controlled differentiation of granulocyte-monocytic precursors ex vivo. ZXDC involvement in the regulation of EGR2 gene expression which posesses the antitumor properties allows to create the basis for the development of new antitumor drugs. Data were generated regarding the process of haemopoethic precursors differentiation and the role of transcriptional factors in the differentiation was analyzed. The area of the application - biomedical institutions.