Lavinskaya E. Study of the role and bioindication of cytotoxic factors in the formation of reversible and irreversible immunophysiological states

The object of study: the cytotoxic factors in blood serum of different nature. Purpose: to characterize the different serum factors with the potential cytotoxic effect and improve the methods of their evaluation on different experimental models. Methods: spectrophotometry, light microscopy, immunoturbidimetry, photometry, electrophoresis of proteins on acetate-cellulose membranes, statistical analysis. Theoretical and practical results, novelty: it was revealed the presence of cytotoxic components in the animal's blood serum, which provided sulfuric copper and the immunized animals, and was shown the dependence of the cytotoxicity degree of the animal's blood serum with age. Found a difference in the concentration of cytotoxic factors, which determined the standard methods and changes in cellular bioindicator in transient and persistent irreversible states in the blood serum of patients with different nosologies besides the number and repertoire of cytotoxic factors are increasing with the development of the non-current conditions. For the first time used formulas assessment of the cytotoxicity, characterizing the reversibility and irreversibility of the violations of the physiological functions of the organism, and shows the specificity of formation of responses bioindicator D. viridis (the definite frequency of occurrence of morphological and functional changes in cells D. viridis under the action of the blood serum of patients with different physiological disturbances). For the first time evaluated the ability to use the test-system D. viridis for the study of the pathological process in dynamics for the forecast of the flow, reversibility and the effectiveness of therapy. Field of application and performance: the proposed screening of the repertoire of serum cytotoxic factors allows you to monitor the severity of a pathological condition, the effectiveness of treatment and to determine the toxicity of pharmaceutical preparations in vitro (including immunotropic) with the subsequent extrapolation on the human body.