Kurovska V. Pathogenetic criteria of differentiating the role of NO-dependent mechanisms in case of ischemic and reperfusion damage of the brain.

Object - ischemic and reperfusion damage of the brain; the purpose - has studied the effect of the donor of nitric oxide of the L-arginine aminoacid on the course of pathobiochemical processes in the hippocampus of male rats under the conditions of incomplete global ischemia of the brain and subsequent reperfusion periods of a diverse duration; methods - biochemical, physiological, statistical; results - it has been demonstrated that the level of nitric oxide metabolites increases under the conditions of 20-minute ischemia and 1- and 24-hour reperfusion, the introduction of L-arginine is conducive to a still larger increase of these indices which reach maximum values under 24-hour reperfusion in all the fields of the hippocampus;a stimulation of the synthesis of nitric oxide augments the tissue fibrinolytic activity in all the hippocampal fields both in case of ischemia and during reperfusion periods, as to the tissue proteolytic activity there occurs an augmentation of the lysis of low- and high molecular weight proteins under the conditions of introducing L-arginine;еhe donor of nitric oxide diminishes the degree of an increase of the products of lipid peroxidation during both reperfusion periods. L-arginine does not influence on the activity of the antioxidant enzymes of superoxide dismutase and catalase, whereas it enhances the activity of glutathione peroxidase, тitric oxide stimulates the pentose phosphate pathway of the glucose exchange under the conditions of ischemia-reperfusion via an activation of its key enzyme - glucose-6-phosphate dehydrogenase.