Prystay M. Biotechnology of surfactants synthesizes by bacteria of the genera Gordonia and Rhodococcus

The thesis is devoted to the development of the technology of G. rubripertincta UKM Ac-122 and R. ruber UKM Ac-288 surfactants as well as the prospects of their practical use. It was established that the G. rubripertincta UKM Ac-122 strain is an active producer of cell-bound surfactants, carotenoids and exopolysaccharide complex. It was shown that G. rubripertincta UKM Ac-122 surfactants were low toxic at the concentrations in the range 0.1-0.5 g/dm3 and contributed to the increase in the permeability of cell membranes of microorganisms belonging to different taxonomic groups. The optimum composition of growth media with the help of mathematical modeling as well as the optimized cultivation conditions for the synthesis of surfactants and EPС by strain G. rubripertincta UKM Ac-122 was developed. The stimulating effect of aspartic acid on the synthesis of glycolipids was shown for the first time. The optimization of extraction of surface-active products from culture R. ruber UKM Ac-288 was carried out using the multiparameteric linear equations. The technology of biosynthesis of surfactants and the EPС by strain G.rubripertincta UKM Ac-122 in fermenter with vortex system of aeration was developed, thus allowed obtaining 10.0 g/dm3 of cell-bound surfactants and 9.2 g/dm3 of EPС. The technology scheme for production of these products was proposed. The perspective of the obtained surface-active products for the practical application as growth promoters of oil plants and for the increase of their resistance to petroleum contamination, as well as for creation of complex biocidal products was shown.