Grytsenko N. Development of technology of surface-active substances of Nocardia vaccinii K-8

The dissertation thesis is devoted to a developing technologies of surface-active substances (SAS, surfactants) of Nocardia vaccinii K-8 with the use of different carbon substrates and studding possibilities of their practical use in environmental technologies. It was determined that the chemical composition of surfactants synthesized by N. vacсinii K-8 on glycerol was a complex of neutral, glyco- and aminolipids. Neutral lipids are represented by mycolic and n-alkanoic acids, glycolipids - by trehalosediacelates and trehalosemycolates. Simultaneous addition of 0,1 % of fumarate (the precursor of gluconeogenesis), and 0.1 % of citrate (regulator of lipid synthesis) into a medium with glycerol in the early stationary growth phase of strain K-8 was accompanied by increase of conditional surfactant concentration by 40 % compared with the cultivation of bacteria in the medoum without organic acids. The composition of nutrient medium for the cultivation of N. vaccinii K-8 was optimized with the use of mathematical methods of experimental planning, which provided maximal indexes of surfactants' synthesis by N. vaccinii K-8. It was shown that the concentration of surfactants synthesized by strain K-8 on glycerol increased by 4 fold (up to 12,6 g/dm3) compared to the indexes before optimization. Our experiments have shown that effectiveness of remediation of oil polluted water (2,6 g/dm3) after a single treatment with a suspension of N. vaccinii K-8 cells was 95 % on the 30th day of exposition.The degree of oil degradation in contaminated soil (20 g of oil per kg of soil) was 85 % at 30 day after treatment with surfactants' preparation of N. vaccinii K-8 as native culture liquid (300 cm3 per 1 kg of soil).