Sushko J. Experimental study of enteric oxygenation in the complex treatment of necrotizing pancreatitis.

Object - experimental of necrotizing pancreatitis; the purpose - dissertation is devoted to the studying of a role of hypoxia in the pathogenesis of necrotizing pancreatitis and pathogenetic substantiation of a combined use of prolonged intragastric oxygenation and peritoneal dialysis in its correction.; methods - physiopathologycal, functional, biochemical, imunological, morphological, mathematicaland the results -found that in response to injury increased lipid peroxidation, decreased enzyme and nonenzyme branches of system of antioxide protection, reaching critical values for 3 days .Until 7 days most of the indices changes for normalization, but does not reach the control level. Increase of the value of indicators of endogenous intoxication (for 3 days and decline to 7 days) and cytolysis (an increase from 1 to 7 days against injury of II and III degree of severity) with a clear dependence on the severity of the injury in the early post-traumatic period was found. Content of CIC in serum in relation to the control group is increased by the 1st day and remained elevated till the 7th day. For injuries of other severity dynamics of content of CIC is variable. Absorption-excretory function of the liver (except for trauma of I degree of severity) content of total and direct bilirubin in bile is reduced regardless of the severity of injury to 3 days; return to the level of control on the 7th day, moreover the amplitude of trauma of II and III degree significantly are higher. Structural deviation of liver tissue in all groups of animals manifests at 3 days after injury simulation and is characterized by acute circulatory disorders, disturbance of structural organization of particles and degenerative-necrotic changes in cells of centrolobular zone and the central part of the hepatic lobule. Symptoms of cholestasis in lobular bile capillaries were more expressed against injury of III degree of severity. In response to the trauma of varying severity observed significant stimulation of apoptosis and necrosis of liver macrophages, which is proportional to the severity of the injury and reaches a maximum of 3 days. Thus against trauma of III degree of severity about 1/3 of liver macrophages are in a state of apoptosis and necrosis.