Konon A. An improvement of the technology for the synthesis of the surface-active substances by Acinetobacter calcoaceticus ІМV В-7241

The dissertation thesis is devoted to аn improvement of the technology for the synthesis of the surface-active substances (SAS) by Acinetobacter calcoaceticus ІМV В-7241 from ethanol and other carbon sources, and also for the study of practical application of SAS with different level of purification. Addition of citrate and fumarate (0.01 %) at the end of exponential phase of A. calcoaceticus ІМV В-7241 growth in the medium with ethanol (2 %) and the maintenance of neutral pH by KOH solution increased the synthesis of SAS in 3.5 times (up to 6.0 g/dm3). The quantity of extracellular SAS synthesized by the strain ІМV В-7241 in the medium containing mixture of n-hexadecane and glycerol (molar ratio 1:7) and C/N 30 increased in 2.6-3.5 times in comparison with cultivation on corresponding monosubstrates. Destruction of oil pollutants of water in complex with heavy metals (3.0-6.0 g/dm3) and soil (20.0 g/kg) by treatment with SAS of A. calcoaceticus ІМV В-7241 in form of culture liquid was 85-98 %. It was shown that preparations of SAS A. calcoaceticus ІМV В-7241 with different level of purification (0.0023-0.36 mg/сm3) decreased the survival of some yeasts and bacteria (including hytopathogenic ones) by 50-100 % and quantity of cells attached to the different abiotic surfaces by 48-98 %.