Bandrivska O. Features of heart cholinergic regulation at streptozotocin-induced diabetes mellitus according to gender (experimental study)

Object - experimental diabetes; the purpose - to define features cholinergic processes in heart in dynamics of development streptozotocin-induced diabetes depending on a gender, methods - functional; biochemical; morphological; mathematical; results - dissertation is devoted to determination of sexual characteristics of diabetic cardiomyopathy development in the experiment. Established that streptozotocin (60 mg/kg) causes different by degree of manifestation deviation of carbohydrate metabolism in male and female rats in the dynamics of development of diabetes mellitus. Content of glucose and glycated hemoglobin in the blood, accumulation in the myocardium ventricles of pyruvate acid, lipid peroxidation products and exhaustion of antioxidants system in the early stages of observation (after 30 days) is more substantial in male rats and from 60 day - in females, which reduces the difference between animals in progression of diabetes. About development of diabetic cardiomyopathy on the microscopical level indicate disturbances in the system microhemocirculation, damage of contractile apparatus and necrosis of cardiomyocytes, proliferative-sclerotic changes the degree of which increases with increasing period of manifestation of diabetes mellitus in males is more pronounced in the early stages of observation (30 days) and in females - in a remote time (60 days). This maintains the difference between animals, indicating intense metabolic and structural abnormalities in the myocardium of males. Changes in autonomic regulation of the heart under these conditions are manifested by weak reactivity of both links - adrenergic and cholinergic. The degree of violations of cholinergic processes in the heart in the conditions of chronic hyperglycemia is more pronounced in females, as evidenced by a significant accumulation of acetylcholine in the presynaptic part of vagus nerve and violation of acetylcholine metabolism in myocardium of atriums and ventricles, reducing the sensitivity of cholinergic receptors.