Kolodnytska G. Pathogenetic aspects of the development and treatment of generalized periodontitis associated with diabetes mellitus (experimental study)

Object - inflammatory diseases of a periodontal tissues on a background diabetes mellitus; the purpose - to investigate biochemical and immune mechanisms of defeat of periodontal tissues by gram-negative microflora endotoxin at animals with a diabetes and to prove effective approaches to integrated corrections of metabolic infringements; methods-immune, biochemical; mathematical; the purpose - It has been shown that lipopolisaccharide periodontitis is accompanied with activation of oxidative and nitrooxidative stress in periodontal tissues and serum, enhanced production of proinflammatory cytokines, increased serum levels of immunoglobulins A, M, G and circulating immune complexes, and activation of degenerative and destructive processes in periodontal connective tissue (alkaline phos-phatase activity is reduced and acid phosphatase activity is increased, reaction of glycoproteins and proteoglycans depolymerization are activated, collagenolysis is enhanced, content of C-reactive protein is increased). The concomitant diabetes mellitus significantly impairs almost all studied parameters in animals with periodon-titis. Antioxidant PEG-SOD, immunomodulator Likopid and iNOS inhibitor Aminoguanidine partially prevent the activation of free radical reactions, overproduction of NO, enhanced catabolism of connective tissue components and inhibit the pro-inflammatory cytokines formation in experimental animals with periodontitis and diabetes. Nonselective NO synthase inhibitor N-nitro-L-arginine stimulates the oxidation processes in periodontal tissues and does not affect significantly the state of the connective tissue in rats with combined pathology. The use of "PEG-SOD+Likopid+Aminoguanidine" combination in periodontitis on the diabetes background is significantly more effective than the use of each agent alone.