Yukhta M. Effect of cryopreserved mesenchymal stromal cells on cartilage defect recovery of intervertebral discs (experimental study)

Research object is the course of reparative processes in degenerative cartilage of intervertebral disk in conditions of the local introduction of the allogenous cryoconserved bone-marrow derived multipotent mesenchymal stromal cells. The aim of the thesis is to determine the impact of cryopreserved allogeneic bone-marrow derived multipotent mesenchymal stromal cells on the intervertebral disk degenerative cartilage defect recovery in rats. Research methods: cytological, cytochemical, histological, morphometric, radiological, cell culturing in vitro, light and fluorescent microscopy, cryoconservation (software freezing) and methods of statistical processing of the results. A new model of the degenerative damage of intervertebral discs in rats was developed, which according to the mechanism of development and the nature of the changes corresponds to degenerative processes in the intervertebral discs in humans. Substantiated and experimentally demonstrated the use of cryopreserved suspension of allogeneic bone-marrow derived multipotent mesenchymal stromal cells to optimize the degenerative intervertebral disk cartilage repair. It was first established that the local introduction of cryopreserved suspension of allogeneic bone-marrow derived multipotent mesenchymal stromal cells on gelatin sponge in the dose 0,5х106 cells within 90 days leads to the restoration of histological structure, height, numerical density of fibrochondrocytes and x-ray density of the fibrous ring of the degenerative intervertebral disc.