Goriacha I. Resistance of Saccharomyces cerevisiae yeast and erythrocytes to cold exposure under of oxidative stress conditions

Research objects were the cross-adaptation processes, caused by stress factors of not cold nature to increase the resistance of erythrocytes and yeast Saccharomyces serevisiae to cold exposures. The research aim was in terms of the yeast Saccharomyces cerevisiae and erythrocytes to determine the relationship between the degree of stress impact on cells and an adaptive response to stress, manifested in an increased cell resistance to cold exposures. Research methods: chemiluminescence, microscopy, flow cytometry, spectrophotometry, spectrofluorimetry, differential scanning calorimetry, methods of statistical analysis.For the first time in terms of the yeast S. cerevisiae there has been experimentally established the fact that an antioxidant capacity is the limiting level of oxidative stress, below which it represents an alarm for cells, and physiological response of cells consists in triggering protective mechanisms not only against oxidative stress, but other stress types as well, including cold one. It was demonstrated that the augmentation of cell resistance to cold exposures via inducing in them of cross-adaptation effects might be achieved by means of various stress agents such as: a strong oxidizer (ozone) or biologically active substances within ASD-2. In this case the dependence of the effect of stress level has qualitatively similar manifestation for different cells and various stress agents, namely the U-shaped form, characteristic for hormetic response of living system to a weak stress. There has been designed, manufactured and patented the bioluminometer, which has wider possibilities to analyse the luminescent signals as compared to the known models. There has been developed and patented the cryopreservation medium for bovine erythrocytes.