Lychkovska N. Activational-enzymatic and phenotypic peculiarities of lymphocytes in patients with inflammatory disorders of the joints.

The dissertation is devoted to the study of cell-mediated immunity and pathogenic peculiarities of inflammatory joint diseases involving arginase-NO-synthase and ATPase systems of blood lymphocytes. It has been found more expressed changes of helper cytotoxic cells (CD4+ and CD8+), activated (CD3+/HLA-DR+), suppressive (CD4-/CD25+) T lymphocytes and B lymphocytes (CD19+) and regulatory cells (CD4+/CD25+) in rheumatoid arthritis patients compared with patients with ankylosing spondylitis, indicating the presence of more powerful immunodependent autoagression in rheumatoid arthritis. The significant increases in arginase activity and iNO-synthase activity and decreases in eNO-synthase activity of blood lymphocytes of patients with rheumatoid arthritis and ankylosing spondylitis in comparison with practically healthy donors have been shown. The significant reduction in Ca2+, Mg2+-ATPase and Na+, K+-ATPase activity of peripheral blood lymphocytes has been shown. It has been found that in conditions of rheumatic pathology the increase of arginase activity in immunocompetent cells was related to the increase of maximum reaction rate. It has been established that inhibition of eNOS occurs by reducing the enzyme reaction speed. It has been found that inhibition of Ca2+, Mg2+-ATPase systems occurs in mixed type and was related to the reduction of maximum reaction rate and to the decrease in Ca2+, Mg2+-ATPase affinity to ATP. It has been found that the inhibition of Na+, K+-ATPase activity was related to the decrease of Na+, K+-ATPase affinity to ATP.