Melnychenko Y. Biotechnology of receiving probiotic supplements and its application in broiler chickens growing

Potentially probiotic strains of Lacto- and Bifidobacterium effect on functional and metabolic status of macrophage activation and interferon synthesis in the blood and splenocytes were studied by screening. For a number of criteria reflecting multifunctionality of the strains action (macrophages activation, cell wall rigidity, holesterol modulating activity) we have selected the most effective, in terms of their potential, strains (B. animalis VKB and L. casei IMV B-7280) and obtained Lactokas and Probifid probiotic medicines on the base of these strains. Technological parameters for their preparation, control methods and quality control have been developed. It has been found out that applying these medicines improves protein metabolism indices as well as an important component non-enzyme chain of antioxidant protection and redox systems of the body, i.e. thiol-disulfide system: it enlarges the number sulfohydride groups (НS-) and increases the thiol-disulfide ratio (НS-/-SS-) . Adding the medicines causes compensation in bifidobacteria and lactobacilli number changes in broilers gastrointestinal tract (gizzard, small intestine, cecum and rectum) which is observed in immunesuppressive periods of broiler chickens post embryogenesis. It has been investigated that cholesterol (r<0,05) and triacylglycerols (r<0,05) concentration decreases reliably in broiler chickens meat and blood under adding probiotic preparations and improves significantly the poultry products nutrition quality and increases its competitiveness. Applying Laktokas and Probifid probiotic preparations in chickens farming enhances their growth intensity, increases live weight by 6,8 %, and the chickens surviving rate by 3,7%. Keywords: poultry, probiotic strains, lactobacilli, bifidobacteria, interferon, imunomodulating traints, probiotic medicines, performance, surviving rate.