Bespalova I. Peptidic composition and biological action of extracts of cryopreserved fragments of pigs' spleen and piglets' skin

Research object was the composition and biological action of the extracts of cryopreserved fragments of pigs' spleen and piglets' skin. The goal was to determine the effect of cryopreservation conditions of the fragments of porcine spleen and piglet's skin on the composition of the extracts derived from them as well as biological effect of the extracts in fibroblasts' culture and in rats with cold trauma of skin. The methods used were as follows: spectrophotometry, spectrofluorimetry, high performance gel permeation chromatography, matrix-assisted laser desorption/ionization, light microscopy, planimetrics, chemiluminescence, statistics. Results. The findings suggest that the yield of peptides from the fragments cryopreserved with PEO-1500, was higher versus either those non-cryopreserved or cryopreserved under glycerol protection. The positive effect of the extracts on the course of wound healing after cold injury of skin and stimulation of fibroblast proliferation activity have been shown. Novelty. It is shown that the molecular weight spectra of peptides being the part of the extract, are tissue specific. For the fist time it has been established that endogenous proteases are important in the formation of peptides when incubating the fragments of organs. It has been demonstrated that the extracts accelerate cold wound healing, herewith they reduce the level of free radical oxidation of lipids and normalize the body's immune system response to cold injury. As well when adding the extracts into the culture medium of rat's skin fibroblasts their metabolic and proliferative activity increased.