Babaieva G. Experimental myocardial necrosis course while application extract of cryopreserved fragments of piglets' heart

Object of study were destructive and regenerative processes in the heart of rats in experimental myocardium cryonecrosis. The goal was to establish the peculiarities of the effect of the extract of cryopreserved fragments of piglets' heart when administered to rats into abdomen on the course of experimental myocardial necrosis. Methods were as follows spectrophotometry, high performance gel permeation chromatography, histology, spectrofluorimetric, ECG, biochemical methods, light microscopy, statistical processing of the research results. Results. Varying the time of cryodestruction of cardiac muscle makes it possible to obtain in the experiment both subepicardial and transmural myocardial necrosis. In animals with subepicardial myocardial necrosis which were introduced with the extract of piglets heart the necrosis diameter was smaller than the control. In experimental animals there was reduced the severity of cytolysis and at earlier terms there were found the e normalization of leukocyte blood counts. In animals administered with the hearextract, the intensity of lipid peroxidation declined faster if compared to the animals with myocardial necrosis. In animals there was increased the number of average weight molecules in blood serum. To day 14 of the experiment in the animals with myocardial necrosis, which were treated with the piglets' heart extract number of average weight molecules were normalized and loading of albumin by ligands in blood serum, whereas in the animals with myocardial necrosis they were statistically and significantly higher than the norm.