Nagorniak I. NO-dependent mechanisms damaging salivary glands of rats under oral cavity exposure to methacrylic acid methyl ester

This dissertation is devoted to investigating the issue, which consists in revealing the impact produced by NO-synthase isoforms as arginase, peroxynitrite and NF-?B on free radical processes and protein synthesis function of submandibular salivary glands (SG) in the oral mucosa of white rats under prolonged exposure to methyl methacrylate. The study has shown the 30-day application of 1 % solution of methyl esters of methacrylic acid onto oral mucosa in the rats causes dysregulations in NO-synthase and arginase metabolic pathways of L-arginine in the submandibulary SG, increases the production of superoxide anion radical, promotes decompensated lipid peroxidation, impairs protein synthesis functions of SG. The differences in the effects of neuronal and inducible NO-synthase under the exposure to action methyl methacrylate have been also found out. The results of the study have demonstrated the introduction of peroxynitrite scavenger of L-selenomethionine and the inhibition of activation of transcription factor ?B by administering an inhibitor of its nuclear translocation JSH-23 (4-methyl-N-(3-phenylpropyl) benzene-1,2-diamine) under the conditions of the experiment is associated with a reduction in the activity of NO-synthase in SG tissues, limited production of superoxide anion radical in the SG tissues by NADH- and NADPH-dependent electron transport chains, the intensity in lipid peroxidation under the increase of antioxidant capacity. It has been newly discovered that the combined administration of L-arginine and JSH-23 under experimental conditions improves the function of SG protein synthesis, normalizes the level of production of superoxide anion radical NADPH- and NADH-dependent electron transport chain, as well as provides an additive effect towards the limiting lipid peroxidation.