Rushchak V. CYP2E1 involvement in the development of pathological processes during metabolic syndrome in guinea pigs

Aim of the work: to determine the role of CYP2E1 in the development of structural and metabolic changes in the liver and pancreas during metabolic syndrome modelling. Object of the research: morphological and biochemical mechanisms of the liver and pancreas tissue damage in the development of metabolic syndrome. Methods: histological, cell-biological, molecular biological and biochemical. The thesis devoted to study of cytochrome P450 2E1 participation in the development of liver damage during experimental metabolic syndrome in guinea pigs. By using of protamine sulfate model of metabolic syndrome it was shown the complex pathological changes in the experimental animals tissues during metabolic syndrome development. The obtained data show a link between the increasing of CYP2E1 expression in liver, increasing the number of free radicals in the blood and development of hepatotoxic processes in animals with metabolic syndrome. It was shown that reducing the activity of CYP2E1 by using of specific inhibitors leads to a decrease of hepatotoxic processes. It was substantiates the possibility of pharmacological regulation CYP2E1 expression and pathological processes that accompany the metabolic syndrome.