Zykova N. Biotechnology of selenium-containing probiotics

The dissertation is devoted to scientific justification and development of technology of getting selenium-containing probiotics and selenium-containing preparats. In the first stage held the selection of optimal source of selenium for its biotransformation by cultures of lactobacilli and bifidobacteria. Such a source is sodium selenite (Na2SeO3). It was determined that the optimal medium accumulation of selenium-containing biomass is laktobacillus medium is a cheese whey. It was defined depressing effect concentrations of sodium selenite 5−20 g / cm3 on the dynamics of accumulation microorganisms. It was investigated the process of assimilation and biotransformation sodium selenite by microorganisms. It was determined a direct correlation between the content Na2SeO3 in the culture medium and the content of organic selenium accumulated by microorganisms. Experiments were performed to determine the survival rate of selenium-containing microorganisms in the conditions of the digestive tract and their antibiotic resistance. Spend a selection of conditions to ensure the destruction of bacterial cells to produce selenium nanostructures. These conditions are the combined effects of concentrated hydrochloric acid, freeze and microwave field action. The method defined pixel size selenium nanostructures. On the basis of the data set selenium dietary supplements from selenium containing probiotics, as well as the preparation of selenium nanostructures. Investigated microbiological, organoleptic and physico-chemical properties of the products obtained.